Over 70% of the transcribed genome is non-coding, including an estimated 58,648 long noncoding RNAs (lncRNAs).  LncRNAs are aberrantly expressed in almost all cancer types and their tissue specific expression makes them attractive therapeutic targets. Functional studies have revealed their role in tumorigenesis stems from active regulation of genes important for cell cycle control, cell survival, and pluripotency. Mechanistically, lncRNAs can function by interacting with transcription factors to modulate target gene expression and with DNA to facilitate chromatin interactions. We are characterizing lncRNA expression in a pediatric cancer cohort (n=1030) comprised of six distinct histotypes using RNA-sequencing from the NCI-TARGET project to determine what role they may play in childhood cancers.  With a multi-omic approach, we integrate epigenomic and  DNA sequencing data to elucidate the regulation of lncRNAs. We will then validate and assess the biological function of lncRNAs, with a primary focus on neuroblastoma. Our goal is to uncover novel lncRNA biology and potentially discover new cancer targets.